Learning about the cutting edge techniques applicable in microbial ecology & microbial physiology from single cells to metabolite analysis. Visualization of cellular activity and processes via molecular and elemental imaging techniques and high resolution microscopy in microbial ecology and physiology. Instrumental and methodological development also fosters these techniques for applications in many other environmental sciences.
Contents
The course covers lectures as well as practical training in:
  1. Sample preparation for chemical microscopy;
  2. Phylogenetic identification of microbial cells by FISH.
  3. Investigation of surface structure and element composition of the samples by high-resolution scanning electron microscopy (SEM) and helium-ion microscopy (HIM).
  4. The spatially resolved secondary ion mass spectrometry (SIMS) techniques including time of flight SIMS (ToF-SIMS) and NanoSIMS for the identification and visualisation of molecular, isotopic, and elemental composition of biological samples.
  5. Ultra-high resolution molecular imaging mass spectrometry using MALDI-FT-ICR MS for spatially resolved biomarker distribution in biological samples (e.g. lipid distribution in zebrafish or rat kidney).
  6. Laser-Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS)
  7. An additional lecture and training will cover the principles of image-processing for correlative microscopy using the image-processing software ImageJ
The exact schedule for the practical trainings will be made after assignments of participants to the techniques.
Didactic Aims
  • overview of the ProVIS available techniques and their application to (micro)- biological field, from single cells to metabolomics approaches
  • introduction to chemical microscopy of (micro)-biological samples from µm to nanometer scale
Didactic Elements
Overview lectures (mass spectrometry and correlative imaging), instrument specific lectures and practical training (1/2 d each) for FISH, LMD, SEM, sample preparation based on chemical fixation, SIMS, LA-ICP MS, MALDI-FT-ICR MS
Target Group
Doctoral Researchers
Postdocs & Senior Scientists
Organisational Info
Date: 07.10.2019 09:00 – 11.10.2019 17:00
Course Duration in Days: 3
Location: UFZ Leipzig: building 4.0, room 101
Course Language: English
Registration Deadline: 07.09.2019
Cancellation Deadline: 20.09.2019
Participation fee for externals (net): 0.00 €

Focus on scientific exchange: Yes
Eligibility for participation: 1.40


Remarks:
Need help? Contact: verena.schaller@ufz.de / 0341 235 1843

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